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    Wisconsin Lawyer
    May 01, 2002

    New Laws Reflect the Power and Potential of DNA (Sidebar)

    Wisconsin's new DNA statutes update the definition of a DNA profile to reflect modern technology: require preservation of and access to biological evidence after conviction; and relax the statute of limitation in sexual assault cases in which a DNA profile of the perpetrator exists. These changes recognize the power of DNA to prove guilt or innocence.

    Keith Findley

    Wisconsin LawyerWisconsin Lawyer
    Vol. 75, No. 5, May 2002

    Standards for Postconviction DNA Testing

    Under Wis. Stat. section 974.07(7)(a), testing is required if:

    1) The movant claims that he or she is innocent of the offense at issue.

    2) It is reasonably probable that the movant would not have been prosecuted, convicted, found not guilty by reason of mental disease or defect, or adjudicated delinquent ..., if exculpatory [DNA] testing results had been available before the prosecution, conviction, finding of not guilty, or adjudication for the offense.

    3) The evidence to be tested meets the conditions under section 974.07(2)(a) to (c) [see below].

    4) The chain of custody of the evidence to be tested establishes that the evidence has not been tampered with, replaced, or altered in any material respect or, if the chain of custody does not establish the integrity of the evidence, the testing itself can establish the integrity of the evidence.

    Section 974.07(2) provides that a person may request DNA testing at any time after conviction, adjudication, or commitment if all of the following apply:

    a) The evidence is relevant to the investigation or prosecution.

    b) The evidence is in the actual or constructive possession of a government agency.

    c) The evidence has not previously been subjected to forensic [DNA] testing or, if the evidence has previously been tested, it may now be subjected to another test using a scientific technique that was not available or was not used at the time of the previous testing and that provides a reasonable likelihood of more accurate and probative results.

    Evolving DNA Profiling Technology

    RFLP - Restriction Fragment Length Polymorphism

    RFLP testing was the dominant form of DNA profiling from the mid-1980s through much of the 1990s. This procedure produced excellent results under the right circumstances. The testing was very discriminating - that is, it could discern unique DNA profiles with great precision, producing results identifying the odds of a random match often in the range of one in several hundred million or even billions. But the procedure had a flaw - it was not very sensitive. That is, results could only be obtained from a fairly sizable sample of biological material, such as a bloodstain about the size of a dime, with DNA that was in good condition. In cases with minute samples, or old and degraded samples, RFLP tests often were inconclusive.

    PCR/STR - Polymerase Chain Reaction Using Short Tandem Repeats

    RFLP has been replaced in recent years by PCR analysis using STRs. PCR is a DNA replicating technique that allows testing on samples that would have been insufficient for RFLP analysis. STRs are short segments of DNA that differ from person to person, and can be used to create a very discriminating profile. Following the lead of the FBI, 13 core loci, or STR segments, have been established in this country as a standard for DNA profiling and databanking. Using these STRs in combination, DNA profiles are both highly sensitive - capable of obtaining results from minute or degraded samples - and remarkably discriminating - capable of producing odds of a random match often in the range of one in many trillions or even quadrillions - far more than the entire population on Earth.

    Mitochondrial DNA Testing

    A third type of testing has emerged in recent years that is being used increasingly in criminal cases - mitochondrial DNA (mtDNA) testing. Most DNA testing relies on DNA drawn from the nucleus of a cell. But some biological material, such as hair shafts or bone, does not have a nucleus and hence no nuclear DNA. Until recently, it was impossible to do DNA testing on such material (testing on hairs could only be done if the hair still had a root, which has nucleated cells). Now, mitochondrial testing can get results from nonnucleated cells, by analyzing the DNA in the mitochondria of such cells. The mitochondria, however, do not contain the full complement of DNA; mitochondrial DNA includes only the DNA inherited from the mother. Mitochondrial DNA testing is essentially a single locus test and cannot discriminate between family members in the line of descent; therefore it is not as discriminating as nuclear DNA. Nonetheless, it can produce probative results, and if the analysis produces an exclusion, that result is conclusive.


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